Composite

Part:BBa_K5246045:Design

Designed by: Edgaras Zaboras   Group: iGEM24_Vilnius-Lithuania   (2024-09-24)


C.Crescentus CB2/CB2A hfsF-hfsC-hfsI Part of polysaccharide export apparatus


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 2813
    Illegal PstI site found at 1121
    Illegal PstI site found at 2620
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 1121
    Illegal PstI site found at 2620
    Illegal NotI site found at 2293
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 2039
    Illegal BglII site found at 2411
    Illegal BglII site found at 2816
    Illegal BglII site found at 3872
    Illegal BamHI site found at 181
    Illegal XhoI site found at 3286
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 2813
    Illegal PstI site found at 1121
    Illegal PstI site found at 2620
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 2813
    Illegal PstI site found at 1121
    Illegal PstI site found at 2620
    Illegal NgoMIV site found at 431
    Illegal NgoMIV site found at 539
    Illegal NgoMIV site found at 821
    Illegal NgoMIV site found at 878
    Illegal NgoMIV site found at 922
    Illegal NgoMIV site found at 953
    Illegal NgoMIV site found at 1177
    Illegal AgeI site found at 239
    Illegal AgeI site found at 3066
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

NA


Source

NA

References