Composite
Part:BBa_K5246045:Design
Designed by: Edgaras Zaboras Group: iGEM24_Vilnius-Lithuania (2024-09-24)
C.Crescentus CB2/CB2A hfsF-hfsC-hfsI Part of polysaccharide export apparatus
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal XbaI site found at 2813
Illegal PstI site found at 1121
Illegal PstI site found at 2620 - 12INCOMPATIBLE WITH RFC[12]Illegal PstI site found at 1121
Illegal PstI site found at 2620
Illegal NotI site found at 2293 - 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 2039
Illegal BglII site found at 2411
Illegal BglII site found at 2816
Illegal BglII site found at 3872
Illegal BamHI site found at 181
Illegal XhoI site found at 3286 - 23INCOMPATIBLE WITH RFC[23]Illegal XbaI site found at 2813
Illegal PstI site found at 1121
Illegal PstI site found at 2620 - 25INCOMPATIBLE WITH RFC[25]Illegal XbaI site found at 2813
Illegal PstI site found at 1121
Illegal PstI site found at 2620
Illegal NgoMIV site found at 431
Illegal NgoMIV site found at 539
Illegal NgoMIV site found at 821
Illegal NgoMIV site found at 878
Illegal NgoMIV site found at 922
Illegal NgoMIV site found at 953
Illegal NgoMIV site found at 1177
Illegal AgeI site found at 239
Illegal AgeI site found at 3066 - 1000COMPATIBLE WITH RFC[1000]
Design Notes
NA
Source
NA